Adapter sets for flexible sample throughput 2 x 24, 2 x 96. Does anyone use the tissuelyser ii qiagen equipment to disrupt organs for virus isolation in cell culture. Guidelines for disruption and homogenization of tissues using the tissuelyser ii 37. Tissuelyser handbook university of texas at arlington. Tissuelyser lt handbook for disruption of up to 12 biological samples. Qiagen offers advanced and innovative solutions for all of your assay and sample equipment needs. Forceps are to be sterilized in fine science tools heat block at. See the tissuelyser lt handbook or the tissuelyser handbook. Place tubes, or collection tube rack, in the respective tissuelyser adapter and operate the instrument according to instructions in the handbook. Tissuelyser ii user manual university of texas at arlington. Qiagen qiacube dnarna purification sample preparation bostonind 09 duration. Servicing of the tissuelyser ii should only be performed by qiagen or an.
All instructions and protocols in this handbook apply to both the tissuelyser ii. For efficient disruption of up to 48 or 192 samples in parallel on the tissuelyser ii. Qiagen, provides free recycling of its weeemarked electronic equipment in. The tissuelyser ii and tissuelyser adapter set 2 x 24 allow highthroughput, rapid, and effective disruption of 48 biological samples in 24 minutes. For disruption of up to 48 or 192 biological samples. Tissuelyser adapter set, 2 x 24 or tissuelyser adapter set 2 x 96 available separately 85300 tissuelyser adapter set 2 x 24. Dna isolation with qiagen genomictips this protocol applies to. Used qiagen mm300 tissuelyser shaker mixer grinder agitator. Complete disruption and homogenization may not always be possible. Does anyone use the tissuelyser ii qiagen equipment to disrupt. May 2016 tissuelyser ii user manual san francisco state.
What is the alternative for tissuelyser for rna extraction from muscle samples. The tissuelyser ii simultaneously disrupts multiple biological samples through high speed shaking in plastic tubes with stainless steel, tungsten carbide, or glass beads. Qiagen kit handbooks and user manuals are available at. Safety information 6 tissuelyser ii user manual 042010 with accessories approved by qiagen see section 3. Place a stainless steel bead 5 mm diameter in the microcentrifuge tube containing the sample. Qiagen qiagility for rapid, highprecision automated pcr setup. Automated nucleic acid purification on the qiacube purification of dna using the dneasy powersoil pro kit can be automated on the qiacube. Storage the tissuelyser should be stored upright in a dry environment at room temperature 1525c. Quality of barley genomic dna extractions using silica powder and different combinations of selfmade. Centrifuge the plate at room temperature for 6 min at 4500 x g. Can the tissuelyser by qiagen be used to lyse cell culture rather than using trizol and the like. Disrupt and homogenize the ffpe core using the tissuelyser ii. Cuttingedge solutions for your life science, applied testing. It has been stored under unfavorable conditions for a prolonged period.
This small bead mill has high throughput with highspeed shaking for the disruption or homogenization of samples in 2 ml microcentrifuge tubes with stainless steel or glass beads. Page 5 l low cost dna extraction for use in tilling and ecotilling assays, version 1. Convenient and secure disruption process adapter sets optimized for highthroughput disruption wide range of accessories available reproducible results with difficulttolyse tissues qiagen tissuelyser ii. Tissuelyser ii tissuelyser ii delivers medium to highthroughput disruption of up to 2 x 96 samples of tissue, plant material, yeast or bacteria in less than 5 minutes. What is the alternative for tissuelyser for rna extraction. Qiagen focuses on breaching great success and development in their company to better serve their consumers. Jun 02, 2014 add the appropriate volume of buffer rlt see table 8, and homogenize by passing the lysate at least 5 times through a blunt 20gauge needle fitted to an rnase free syringe. There are minor cosmetic scratches on the top plate, but nothing serious. Place the tube containing the homogenate on the benchtop at room temperature 1525c for 5 min. Using the appropriate adapter set, up to 48 or 192 samples can be processed at the same time. Tissuelyser ii, supplied by qiagen, used in various techniques. Homogenates were subjected to a single freezethaw cycle to aid cell lysis and then cleared by centrifugation at 10,000 x g for 5 min at 4 c.
Print bookmark share more for disruption of up to 12 biological samples. Qiagen disruption systems for a range of throughputs automated solutions from sample to result. Laboratory equipment qiagen qiacube connect safety instructions and quick start manual 56 pages for use with qbase laboratory equipment qiagen tissuelyser ii user manual 48 pages. Laboratory equipment qiagen tissuelyser ii user manual 48 pages. The tissuelyser ii simultaneously disrupts multiple biological samples through highspeed shaking in plastic tubes with stainless steel, tungsten carbide, or glass beads. It has been subjected to severe transport stresses. The innovative qiacube uses advanced technology to process qiagen spin columns. Does anyone use the tissuelyser ii qiagen equipment to disrupt organs for virus isolation. Prepared by bernhard hofinger, owen huynh and brad till. Tissuelyser adapter set 2 x 96 from qiagen selectscience. Do not continue to use the tissuelyser ii or tissuelyser accessory if it is damaged.
The qiagen tissuelyser lt sample disruptor provides quick and efficient disruption in up to 12 samples. Qiagen tissuelyser ii sample disruption 11843 youtube. Dna adsorbs to the dneasy membrane in the presence of high concentrations. Before using the tissuelyser ii, it is essential you read this user manual carefully. The tissuelyser lt is fully compatible with qiagen manual sample preparation kits. Sample tubes rb 2 ml x 2 ml sample tubes, for tissuelyser lt qiagen 990381 reagent dx qiagen 19088 allprep kit mini qiagen 80204 allprep kit micro qiagen 80284 fisherbrand textured nitrile gloves large fisher 27005853 ice bucket green fisher 1167636 wet ice in house na na nuclease free 2. The tissuelyser ii is also compatible with qiagen manual sample. Low cost and nontoxic genomic dna extraction for use in molecular marker studies.
If the tissuelyser ii is not in use, switch it off at the power switch. Objective to develop and adapt a method for low cost and nontoxic genomic dna extraction for use in molecular marker studies. Tissuelyser ii medium to highthroughput disruption. Does anyone use the tissuelyser ii qiagen equipment to. Tissueruptor tissuelyser lt tissuelyser ii throughput samplesrun 1 up to 12 up to 48 or 192 disruption technology rotorstator bead mill bead mill disruption time 30 seconds 40 seconds to 5 minutes 20 seconds to 5 minutes table 1. Isolate a suitable piece of tissue and place in a uvcrosslinked 1. For longer storage, qiaquick spin kits can also be stored at 28c, but in this case the buffers should be redissolved before use. Equipnet is constantly receiving used qiagen equipment through our exclusive contracts with our clients. Lowcost dna extraction for use in tilling and ecotilling assays. Tissuelyser lt handbook qiagen gel electrophoresis.
Does anyone use the tissuelyser ii qiagen equipment to disrupt organs for virus. The tissuelyser ii is an integral part of qiagen s complete solution for sample management from sample collection to purification and analysis of dna, rna, and protein. Tissuelyser ii user manual 052016 7 it or the line power cord appears to be damaged. Does anyone use the tissuelyser ii qiagen equipment to disrupt organs for. Alternatively, a grinding jar set can be used to process large samples. Low cost and nontoxic genomic dna extraction for use in. Can the tissuelyser by qiagen be used to lyse cell culture. Welcome to qiagen digital insights labcorp uses qci and hgmd to improve identification and interpretation of genetic variants within inhereited diseases. All instructions and protocols in this handbook apply to both the tissuelyser ii and the tissuelyser. Advanced settings appendix f advanced settings advanced run settings are available to adjust specific functions on the qiacube ht. Simultaneous purification of genomic dna and total rna, including small rnas from ffpe cores, using the allprep dnarna ffpe kit. Total rna extraction from tissues for microrna and target. This german made shaker appears in good condition without cracks in the plastic cover or other damage to the housing or workings.
The tissuelyser ii is an integral part of qiagens complete solution for sample management from sample collection to purification and analysis of dna, rna, and protein. We stiil use pestle and mortar which is very timeconsuming and we would like to speed. Reorient plates so that the side that was closest to the machine body is now farthest from it and shake again at speed 20 hz for 10 min. Cignal lenti reporter handbook sabiosciences, a qiagen. Do not continue to use the tissuelyser ii or tissuelyser. The following guidelines can be used for disruption and homogenization of. Use of the tissuelyser ii with nonqiagen adapters has been tested by other. Homogenization and disruption with the tissuelyser ii gives results comparable to using rotorstator homogenization.
It ramps up to 30 shakes per second and the timer works as it should. Does anyone have experience with tissue lyser from qiagen. Apr 28, 2017 tissuelyser lt tissuelyser lt is a small bead mill that enables low to mediumthroughput sample disruption, of up to 12 samples at the same time. For highthroughput disruption of biological samples pdf 89kb. This easytouse instrument automates purification of dna, rna, and protein from 196 samples. Qiagen s position as the market leader for sample and assay technologies is based on more than 25 years of experience, strong relationships with customers, o. The tissuelyser ii complements qiagen automation for highthroughput sample preparation and analysis see table qiagen highthroughput automation, such as the qiasymphony sp. Disruption and homogenization using the tissuelyser lt or tissuelyser ii. In the european union, in accordance with the specific weee recycling requirements and where a replacement product is being supplied by qiagen, free. Qiaquick spin kits should be stored dry at room temperature 1525c.